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Lonicerae flos aqua-acupuncture solution(LFAS) and Lonicerae flas water-extracted solution(LFWS) were prepared and tested for their organ toxicities, potential antitumor activities, and chemopreventive potentials. The organ-toxicity of LFAS to male ICR mice was studied by the measurements of GOT, GPT, LDH, and ALP-s activities after injection of LFAS for 7 days. The activities of GOT, LDH and ALP-s were decreased with LFAS. The activity of GPT was not changed with LFAS. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of LFAS at 10 × and 5 × resulted in more than 60% inhibition of growth in Ehrlich ascites tumor cells(EATC), Hepalclc7, and HeLa cells. Toxicil:y of LFAS to A549 revealed that 82% inhibition of growth at concentration of 10 ×. Concentration of LFWS at 10 × and 5 × showed more than 50% inhibition of growth with EATC, Hepalclc7, HeLa and A549. In morphological study, the number of cells were decreased, and the shape of cells was round-form in EATC, Hepalclc7, HeLa, and A549 with LFAS. Mice given only Ehrlich cells survived an average of 0% after 20 days, but mice given Ehrlich cells and 1 × LFAS had 50% survival. Administration of LFAS(1 × /day) inhibited the growth of Ehrlich ascites cells in vivo. It was used three biomarkers(quinone reductase, ornithine decarboxylase, glutathione) to test chemopreventive potentials of LFAS. LFAS was potent inducer of quinone reductase activity in Hepalclc7 murine hepatoma cells in culture, whereas LFWS is less potent, LFAS and LFWS were significantly induced quinone reductase activity in cultwed human hepatoma Hep3B cells. The effects of LFAS and LFWS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba arstellanii in a broth medium was inhibited by LFAS and LFWS at concentrations of 0.1 ×, 0.5 ×, 1 ×, and 3 ×. In addition, glutathione levels were increased about 2-fold with LFAS and 1.5-fold with LFWS in cultured murine hepalclc7 cells. LFAS and LFWS were also shown to increase glutathione levels in human Hep3B cells. These results suggest that LFAS has antitumor activity in uitro and in vivo. LFAS has chemopreventive potential by inducing quinone reductase activity, inhibition of ODC activity and increasing GSH levels.
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